Cryo- Electron Tomography

Dr Amélie Leforestier (DR CNRS, LPS, Université Paris-Saclay)
  • Quand ? Le 13/10/2021, de 16:00 à 17:00 (Europe/Paris / UTC200)
  • Où ? Webinar
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The principles and typical workflow of cryo-electron tomography will be presented, including: missing information, tilt series acquisition, phase plates and energy filtering, tilt series alignment and tomogram reconstruction and visualization.

Amélie Leforestier is a biophysicist and cryo electron microscopist head of the team Structure and dynamics of self-assembled biological objects at the Solid state Physics Laboratory (LPS, Université Paris-Saclay). She studied biophysics at UPMC (Paris) and obtained a PhD for her work on liquid crystalline phases of DNA. Supported by an EMBO fellowship, she joined Jacques Dubochet's lab in Lausanne, where she discovered cryo electron microscopy in the 90's. Back in France, she joined the CNRS at LPS, where she set up a cryo-EM lab dedicated to soft matter physics and physics of biological systems. Her research interests focus on the architecture of DNA and chromatin assemblies in vitro and in situ.

Online presentation (pdf format)