Webminar: A multi-approach benchmarking of macromolecular interactions - Focus on kinITC

Summary: In recent years, numerous innovative biophysical methods, such as MST, BLI, and switchSENSE, have emerged for studying molecular interactions. While these technologies offer new possibilities, they also raise questions about how their results compare to established techniques like SPR and ITC microcalorimetry. Developers of these new methods often provide limited direct comparisons, leaving the scientific community to grapple with discrepancies between techniques. A recurring question arises: "Why do the results obtained using technique X differ from those generated by technique Y on the same system?". To address this, I will present results from a rigorous comparison of multiple methods, with an emphasis on the kinITC approach developed in our laboratory. This technique extends ITC's capabilities to include kinetic data alongside traditional thermodynamic measurements, offering a more comprehensive analysis of molecular interactions.