Session 2019: AILM Practical school on Advanced Isotopic Labeling for NMR

The practical School is organized in Grenoble just after AIML2019 conference, from March 29th to April 5th 2019. The training will be primarily based on practical sessions in which challenging biological systems are produced and labelled. Systems such as large multi-domain proteins, large monomeric proteins, oligomeric protein assemblies, and large RNAs will be used as well as RNA and protein constructs brought by the participants. Practical sessions will be linked to theoretical classes, and discussion sessions will be organized to promote exchange between participants and instructors. All labelled samples produced by the trainees will be characterized on the IBS high-field NMR platform.
  • Session 2019: AILM Practical school on Advanced Isotopic Labeling for NMR
  • 2019-03-29T13:30:00+01:00
  • 2019-04-05T13:30:00+02:00
  • The practical School is organized in Grenoble just after AIML2019 conference, from March 29th to April 5th 2019. The training will be primarily based on practical sessions in which challenging biological systems are produced and labelled. Systems such as large multi-domain proteins, large monomeric proteins, oligomeric protein assemblies, and large RNAs will be used as well as RNA and protein constructs brought by the participants. Practical sessions will be linked to theoretical classes, and discussion sessions will be organized to promote exchange between participants and instructors. All labelled samples produced by the trainees will be characterized on the IBS high-field NMR platform.
  • Quand ? Du 29/03/2019 13:30 au 05/04/2019 13:30 (Europe/Paris / UTC100)
  • Où ? Institut de Biologie Structurale, Grenoble
  • Nom du contact Dr Jérôme boisbouvier
  • Téléphone du contact jerome.boisbouvier@ibs.fr
  • Participants 16 participants
  • Site Internet Visitez le site Internet
  • Ajouter l'événement au calendrier iCal

The sessions will cover the following isotopic labeling methods: 

-Adaptation, optimization and large scale production of protein in E. coli grown in perdeuterated media

-Methyl specific labelling of large proteins 

-Cell-free expression and specific labelling of soluble and membrane proteins

-Isotopic labeling of protein produced in eukaryotic cells

-Backbone specific labelling and unlabelling

-In vitro production of specifically labelled RNA 

-Cost effective strategies for in vivo production of uniformally labelled RNA 

-Segmental labelling of proteins & RNAs

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