Webminar: Overview of cell-free protein production & developments for NMR samples
Lionel Imbert, Institut de Biologie Structurale, Grenoble
- https://www.renafobis.fr/seminaires-web-renafobis/webminar-overview-of-cell-free-protein-production-developments-for-nmr-samples
- Webminar: Overview of cell-free protein production & developments for NMR samples
- 2024-11-14T13:00:00+01:00
- 2024-11-14T14:00:00+01:00
- Lionel Imbert, Institut de Biologie Structurale, Grenoble
Le 14/11/2024, de 13:00 à 14:00 (Europe/Berlin / UTC100)
Summary: Cell-free expression is used to produce protein samples for structural biology studies. It uses the translation and transcription machinery of E. coli to synthesize proteins in an open environment, i.e. without the bacterial cell wall. For NMR applications, isotopically-labelled amino acids can be used directly in the reaction to synthesize only the protein of interest. Rapid production and simplified purification processes have enabled many structures to be solved by NMR, but there are a number of limitations. Firstly, the size of the proteins to be studied with uniform labelling cannot exceed 20 kDa. Secondly, for higher molecular weight proteins, a larger quantity of protein needs to be synthesized at reasonable cost. Developments made on the IBS/ISBG cell-free platform to perform perdeuteration in water, selective protonation, therapeutic antibody fragment production and position-specific labelling will be among others presented.